Based on its experience, Oncomedics has on its own a series of resources that can be shared with other private and public laboratories.
Bank of living tumor cells
The Oncomedics bank may provide tumor cells or tumor fragments. These fragments or cells are alive and can then be cultured using the Oncomedics products and procedures developed by Oncomedics. The tumor fragments or cells are placed in a storage medium developed by Oncomedics then frozen and stored in liquid nitrogen tank. Patient associated data for each sample (cancer type, date and time of collection, and clinical pathology results) are listed in a database built by Oncomedics preserving the anonymity of the patient.
Unique series of Optimized media for primary cells:
- Safely transport fresh tumors with OncoMid-Via™ media series specifically developed to safely transport fresh tumor samples.It is crucial that the viability of the fresh tumor sample remains intact during its transport from the hospital to the laboratory. In order to preserve such cell viability, Oncomedics has developed OncoMid-Via™ media series allowing human primary cells 48h following the tumor excision. OncoMid-Via™ media series have been developed for breast, colorectal and ovarian cancers (see table for specific references).
- To dissociate selected cell subtypes, OncoMid-Diss™ media series specifically optimized to dissociate human cancer primary cells. OncoMiD-Diss™ media series allow human tumour sample dissociation to selectively obtain human tumour primary culture. Four different vials have been prepared to specifically initiate, control and stop the tumor dissociation using a procedure specific for each tumor type. OncoMid-Diss™ media series have been developed for breast, colorectal and ovarian cancers (see table for specific references).
- To cultivate primary cultures, OncoMiD™ media series optimized to cultivate dissociated human cancer primary cells. OncoMiD™ media series comprise standardized, serum-free media intended for the preparation of in vitro tumour cell primary cultures from human tumour samples. For example, the OncoMiD™ for breast defined medium (ref. OMCULT1) has been optimized to get, from almost all breast cancer subtype samples, tumour cell cultures within 7 days. During this time lapse, strong cell mortality is observed, reflecting the selective elimination of microenvironment cells. Following this, a heterogeneous breast tumour cell primary culture is obtained. The OncoMiD™ for breast medium works better in combination with: OncoMid-Via™ for breast (ref. OMVIA1) for tumour sample transport; OncoMiD-Diss™ for breast (ref. OMDIS1) for sample preparation and dissociation. OncoMid™ media series have been developed for breast, colorectal and ovarian cancers (see table for specific references).