PerCP-conjugated anti-chicken primary antibodies are affinity-purified antibodies directed against chicken (Gallus gallus) immunoglobulins or specific chicken proteins and covalently labeled with Peridinin-chlorophyll-protein complex (PerCP). PerCP is a phycobiliprotein fluorophore excitable by 488 nm lasers, providing emission in the far-red region. PerCP conjugates are widely used in multicolor flow cytometry panels where a far-red channel with moderate brightness and minimal spill into green channels is required. These one-step reagents simplify workflows by removing secondary antibodies and offering consistent antigen recognition for cytometry and fluorescence assays.
Key Features
- Far-red emission: PerCP emits in the ~675 nm region (instrument-dependent), supporting spectral separation in multicolor panels.
- Direct labeling: Primary antibodies conjugated to PerCP enable one-step detection, reducing assay time and secondary cross-reactivity.
- Optimized conjugation: Controlled dye:protein (D/P) ratios and gentle coupling preserve antibody affinity and fluorophore performance.
- Formats: Available as monoclonal and polyclonal antibodies in liquid or lyophilized forms; custom conjugation services may be available.
- Stabilized formulations: Supplied in buffer systems with stabilizers (e.g., BSA) and preservative options; azide-free variants may support live-cell use.
- Lot QC: Each lot is evaluated for D/P ratio, fluorescence performance (MFI), and functional validation in flow cytometry.
Common Applications
- Flow cytometry (FACS): Used in multicolor panels requiring far-red detection with 488 nm excitation compatibility.
- Multicolor immunofluorescence: Applicable in fluorescence microscopy with appropriate filter sets and detectors.
- Cell sorting: Suitable for sorting workflows when instrument sensitivity and panel design are optimized.
- Plate-based fluorescent assays: Applicable in bead-based multiplex assays and fluorescence plate reader formats.