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Cas12

Cas12

Cas12, also known as Cpf1, is a class of RNA-guided DNA endonuclease that has emerged as a versatile tool in the CRISPR/Cas genome editing landscape. Originally discovered in various bacterial species, Cas12 offers unique properties that distinguish it from the more widely known Cas9 enzyme.

Key Features of Cas12

  • RNA-Guided Mechanism: Like other CRISPR enzymes, Cas12 utilizes a guide RNA (gRNA) to identify and bind to specific DNA sequences. The gRNA directs Cas12 to its target by recognizing a complementary sequence adjacent to a protospacer adjacent motif (PAM), typically characterized by the sequence TTTV (where T can be any nucleotide).
  • Unique Cleavage Activity: Cas12 introduces staggered double-strand breaks in DNA, creating 5’ overhangs that facilitate more efficient homologous recombination compared to the blunt ends produced by Cas9. This feature enhances the precision of gene editing and allows for more effective integration of donor DNA.
  • Self-Processing Capability: One of the notable characteristics of Cas12 is its ability to process its own pre-crRNA into mature crRNAs without the need for additional trans-activating RNA (tracrRNA). This simplifies the preparation and delivery of gRNAs for various applications.

Applications of Cas12

  • Gene Editing: Cas12 is widely used for targeted gene knockout, knock-in, and base editing across various organisms, including plants, animals, and human cells. Its unique properties make it an attractive alternative to Cas9 for specific genomic contexts.
  • Diagnostics: The collateral nuclease activity of certain Cas12 variants enables their use in sensitive diagnostic applications. For instance, Cas12-based assays can detect specific nucleic acid sequences associated with pathogens, facilitating rapid disease diagnosis.
  • Therapeutic Development: Researchers are exploring the potential of Cas12 in therapeutic contexts, such as gene therapy for genetic disorders and cancer treatment. Its versatility and efficiency make it suitable for various therapeutic strategies.

Frequently Asked Questions (FAQ)

How can I quickly find a cas12 product?

To find a cas12 product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Oncomedics stock several product codes in the cas12 category?

Yes, Oncomedics distributes several product references in the cas12 category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for cas12 products?

Yes, you can request a quote for cas12 products from the product page or by contacting Oncomedics (info@oncomedics.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a cas12 product is available to order?

The availability of a cas12 product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Oncomedics with the product code or name of the product you are looking for.

What should I do if I cannot find the cas12 product I am looking for?

If the cas12 product you are looking for does not appear in the catalogue, please contact Oncomedics with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all cas12 products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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