DNA extraction
Nucleic acid extraction methods are essential to molecular biology and are commonly used in many medical and biological science applications.
Nucleic acid extraction can be divided into 3 steps, which can be optimized according to the type of sample and the downstream applications for which the nucleic acids will be used. These steps are as follows:
1) Rupture of tissue and cell structures
2) Removal of proteins, lipids and other contaminants from nucleic acids
3) Transfer the nucleic acids to water or a buffer solution that will preserve them without interfering with subsequent work.
Nucleic acid extraction methods can be classified into two different types :
- Solution methods (such as the phenol-chloroform method)
- Methods based on the solid phase (such as magnetic beads or centrifugation columns).
Solid-phase extraction methods work by causing nucleic acids to bind to solid supports, such as magnetic beads coated with silica or other materials. The beads (or other supports) are then washed with alcohol to remove contaminants. The support is then washed with a liquid that makes the nucleic acids soluble again, which releases the DNA from the support in a process known as elution. A simple method is to add a solution containing a chaotrope to the crude nucleic acid extract. Chaotropic agents are molecules that disrupt the hydrogen bonding network between water molecules. In the presence of chaotropes, nucleic acids are previously much less soluble and bind to glass, silica-covered magnetic beads and other solid supports - allowing them to be easily separated from contaminants. Solid-phase extraction methods may also depend on selective binding of DNA and RNA to ion exchange resins or other chemicals.
Frequently Asked Questions (FAQ)
Where can I find dna products on the Oncomedics website?
dna products are available in this section and can be sorted by product families or sub-categories, such as Genomic DNA. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the dna category?
The dna category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Genomic DNA sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a dna product?
To find a dna product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Oncomedics stock several product codes in the dna category?
Yes, Oncomedics distributes several product references in the dna category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for dna products?
Yes, you can request a quote for dna products from the product page or by contacting Oncomedics (info@oncomedics.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a dna product is available to order?
The availability of a dna product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Oncomedics with the product code or name of the product you are looking for.
What should I do if I cannot find the dna product I am looking for?
If the dna product you are looking for does not appear in the catalogue, please contact Oncomedics with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all dna products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.